Neutrophils diminish T-cell immunity to foster gastric cancer progression: the role of GM-CSF/PD-L1/PD-1 signalling pathway

Neutrophils are the predominant leucocytes in the blood and act as the first line of host defence against invading pathogens. Neutrophils have also been shown to play important roles in the other pathological conditions, including cancer. In the past decade, many efforts have been made to clarify the roles of neutrophils in cancer development and progression. It appears that neutrophils have both antitumour and protumour functions. On one hand, neutrophils can directly kill tumour cells by releasing antimicrobial and cytotoxic contents that are prestored in their granules. Neutrophils can induce apoptosis in tumour cells and reduce tumour growth when administrated into tumour-bearing animals. Neutrophils are regarded as important effector cells for monoclonal antibody (mAb)-mediated immunotherapy, where they interact with mAb through the Fc receptor, leading to antibody-dependent cell cytotoxicity. Neutrophils also have antimetastatic activity. In mouse models of cancer metastasis, neutrophils at the premetastatic site produce cytotoxic substances to eliminate tumour cells and limit their metastatic spread. In addition, neutrophils are able to regulate the activation of T cells and other immune cells to elicit antitumour immune responses. On the other hand, emerging evidence suggests that neutrophils possess protumour properties including the induction of malignant transformation, enhancement of tumour growth, establishment of premetastatic niche, stimulation of angiogenesis and promotion of immune evasion by suppression of innate and adaptive immune cells (eg, T cells and NK cells). The dual roles of neutrophils in cancer might be explained by their plasticity and the existence of distinct neutrophil subsets with differing properties within the tumours, which could be driven by signals from the tumours. 3 In particular, neutrophils seem to have both stimulatory and suppressive roles in T-cell immunity. During the early phase of tumour growth, neutrophils tend to inhibit primary tumour growth by recruiting and activating CD8 T cells. 5 On the contrary, during the late stage of cancer, tumour-derived factors polarise neutrophils to an immunosuppressive phenotype that suppresses antitumour T-cell responses and promotes immune evasion. However, the detailed mechanisms responsible for the modulation of neutrophil function in T-cell immunity in human cancer remain largely unknown. In this issue of Gut, Wang et al identified a new mechanism by which neutrophils suppressed T-cell function and promoted the growth of gastric cancer.By analysing clinical samples, they generated data to show that the number of neutrophils was increased in the tumour tissues of patients with gastric cancer. The neutrophils accumulated in gastric cancer tissues had prolonged survival, displayed an activated phenotype and expressed higher levels of programmed death-ligand 1 (PD-L1), an important co-inhibitory molecule that interacts with programmed death 1 (PD-1) on T cells to block their proliferation and activity. They further demonstrated that the increased infiltration of PD-L1 neutrophils in tumour tissues was associated with disease progression and poor patient survival. In line with the findings observed in tumour tissues, neutrophils isolated from the peripheral blood of healthy donors strongly expressed PD-L1 when primed with tumour tissue-derived culture supernatants. Finally, the authors demonstrated that the activated PD-L1 neutrophils effectively inhibited the proliferation and activity of PD-1 T cells (but not PD-1 T cells) in vitro and dampened T-cellmediated antitumour immune responses to promote gastric cancer growth ex vivo; however, blocking PD-L1/PD-1 interaction by an anti-PD-L1 antibody reversed these effects, indicating that neutrophils promote gastric cancer growth via suppression of T-cell function in a PD-L1/ PD-1 interaction-dependent manner. While looking for the mechanism by which tumours induce the immunosuppressive phenotype in neutrophils, the authors found that tumour cell-derived granulocyte-monocyte colony-stimulating factor (GM-CSF) efficiently induced the expression of PD-L1 on neutrophils through activation of the Janus kinase/ signal transducer and activator of transcription 3 signalling pathway. The work by Wang and colleagues adds new evidence to support the protumour function of neutrophils. These cells seem to promote tumour growth through both direct and indirect mechanisms (figure 1). Neutrophils can produce a wide spectrum of proteinases and inflammatory factors that directly promote tumour cell proliferation, such as neutrophil elastase, prostaglandin E2 and interleukin-1 β (IL-1β). 9 Neutrophils can also promote tumour growth by regulating the tumour microenvironment. Neutrophils are suggested to share similarities with granulocytic myeloid-derived suppressor cells, which exhibit potent immunosuppressive activities in inflammatory diseases and cancer. Neutrophils-mediated immune suppression involves multiple mechanisms, including release of inducible nitric oxide synthase (iNOS) (in mouse), production of arginase 1 (ARG1) (in human) and recruitment of regulatory T cells (Treg) (in human and mouse). In this study, the authors found that neutrophils could suppress T-cell function though an increased PD-L1/PD-1 interaction, which adds a new layer of complexity to the immunosuppressive roles of neutrophils in cancer. They also demonstrated that PD-L1 blockade in neutrophils reversed the inhibition of T-cell function to a greater extent than blocking iNOS and ARG1, suggesting that PD-L1 is the main factor that mediates the immunosuppressive roles of neutrophils in T cells in human gastric cancer. Intriguingly, neutrophils have been reported to inhibit T-cell activity to promote lung metastasis without affecting primary tumour growth in a mouse breast cancer model. Wang et al showed that the neutrophil percentage within human gastric cancer was associated with advanced lymphatic invasion; however, no significant correlation was observed between the neutrophil percentage and distant metastasis. The effect of PD-L1 neutrophils on gastric cancer metastasis was not tested. Thus, whether PD-L1 neutrophils may influence gastric cancer metastasis is a question waiting to be explored. Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China